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Medline-entry ::= {
uid 88055872,
em std { year 1988, month 3 },
cit {
title {
name "Developmental regulation of a constitutively expressed mouse mRNA encodi
ng a 72-kDa heat shock-like protein." },
authors {
names ml {
"Giebel LB",
"Dworniczak BP",
"Bautz EK" },
affil str "Zentrum fur Molekulare Biologie, Universitat Heidelberg (ZMBH), Fed
eral Republic of Germany." },
from journal {
title { ml-jta "Dev Biol" },
imp {
date std {
year 1988,
month 1
},
volume "125",
issue "1",
pages "200-7"} } -- end Cit-jour
}, -- end Cit-art
abstract "Multiple heat shock cognate (hsc70) cDNA clones were isolated from t
he mouse embryonal carcinoma cell line F9. They all encode a single 72-kDa pro
tein, which is constitutively expressed in all mouse cell lines and tissues te
sted, and which is only slightly induced by hyperthermia. hsc70 RNA is very ab
undant in F9 stem cells and brain, but very little is found in 14-day-old embr
yos. Upon differentiation of F9 stem cells induced by retinoic acid and cyclic
AMP, expression of the hsc70 gene decreases only slightly, suggesting that hs
c70 is highly expressed in early mouse development and is then down-regulated
towards the end of embryogenesis. In adult tissues only the brain retains the
high level of hsc70 gene expression found in F9 stem cells. We also show that
expression of hsc70 protein and clathrin is uncoupled in F9 cells, indicating
that the uncoating activity of coated vesicles may not be the only function of
hsc70 protein.",
mesh {
{ term "Amino Acid Sequence" },
{ term "Animal" },
{ term "Base Sequence" },
{ term "Cell Differentiation" },
{ term "Clathrin",
qual { { subh "metabolism" }}},
{ term "Cloning, Molecular" },
{ term "DNA",
qual { { subh "analysis" }}},
{ term "Gene Expression Regulation" },
{ term "Heat-Shock Proteins",
qual { { mp TRUE, subh "genetics" }}},
{ term "Mice" },
{ term "Molecular Sequence Data" },
{ term "RNA, Messenger",
qual { { mp TRUE, subh "analysis" }}},
{ term "Stem Cells",
qual { { subh "cytology" }}},
{ term "Support, Non-U.S. Gov't" },
{ term "Translation, Genetic" }},
substance {
{ type cas, cit "9007-49-2", name "DNA" }},
xref {
{ type genbank, cit "M19141" }}
} -- end Medline-entry
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